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CD99 Monoclonal antibody, PBS Only

CD99 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5B6A4

Cat no : 60354-1-PBS

Print datasheet

Synonyms

12E7, CD99, CD99 antigen, CD99 molecule, E2 antigen, MIC2, MIC2X, MIC2Y, Protein MIC2, T cell surface glycoprotein E2

验证数据展示

  • WB analysis of human spleen using 60354-1-Ig (same clone as 60354-1-PBS)

    WB analysis of human spleen using 60354-1-Ig (same clone as 60354-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 60354-1-Ig (CD99 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60354-1-PBS in a different storage buffer formulation.

产品信息

60354-1-PBS targets CD99 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CD99 fusion protein Ag19474 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD99 molecule
Synonyms 12E7, CD99, CD99 antigen, CD99 molecule, E2 antigen, MIC2, MIC2X, MIC2Y, Protein MIC2, T cell surface glycoprotein E2
Calculated Molecular Weight 19 kDa, 16 kDa, 17 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC021620
Gene Symbol CD99
Gene ID (NCBI) 4267
RRIDAB_2881463
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP14209
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing’s sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917). CD99 displays two surface isoforms generated by alternative splicing: a long 32 kDa and a short 28 kDa form (PMID: 12368226).