验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13732-1-AP targets CD99L2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | CD99L2 fusion protein Ag3809 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CD99 molecule-like 2 |
| 别名 | UNQ1964/PRO4486, MIC2-like protein 1, MIC2L1, CD99B, CD99 antigen-like protein 2 |
| 计算分子量 | 262 aa, 28 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC025729 |
| 基因名称 | CD99L2 |
| Gene ID (NCBI) | 83692 |
| RRID | AB_3669170 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TCZ2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD99L2 (CD99 molecule like 2), also known as CD99B and MIC2L1, is a type 1 transmembrane protein that is similar to CD99 (PMID: 12706889). It is expressed on most leukocytes and several other cell types including neuronal cells (PMID: 12706889). CD99L2 may function as a homophilic adhesion molecule as well as play a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane (PMID: 37199607). Its related pathways respond to elevated platelet cytosolic Ca2+ and cell surface interactions at the vascular wall.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD99L2 antibody 13732-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |