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  • Featured Product
  • KD/KO Validated

CDC123/C10orf7 Polyclonal antibody

CDC123/C10orf7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 28178-1-AP

Print datasheet

Synonyms

CDC123, C10orf7, Protein D123, HT-1080, HT 1080

验证数据展示

  • WB analysis using 28178-1-AP

    WB analysis using 28178-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 28178-1-AP (CDC123/C10orf7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 28178-1-AP

    IF Staining of HeLa using 28178-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28178-1-AP (CDC123/C10orf7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach using 28178-1-AP

    IHC staining of human stomach using 28178-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28178-1-AP (CDC123/C10orf7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28178-1-AP targets CDC123/C10orf7 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen CDC123/C10orf7 fusion protein Ag26828 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 123 homolog (S. cerevisiae)
Synonyms CDC123, C10orf7, Protein D123, HT-1080, HT 1080
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 35-39 kDa
GenBank Accession NumberBC001600
Gene Symbol CDC123
Gene ID (NCBI) 8872
RRIDAB_2881081
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75794
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDC123/C10orf7 antibody 28178-1-APDownload protocol
IHC protocol for CDC123/C10orf7 antibody 28178-1-APDownload protocol
IF protocol for CDC123/C10orf7 antibody 28178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,CoIP

Pathol Res Pract

CDC123 promotes Hepatocellular Carcinoma malignant progression by regulating CDKAL1

Authors - Yong Wang
  • KD Validated
View Article