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CDC16/APC6 Polyclonal antibody

CDC16/APC6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14307-1-AP

Print datasheet

Synonyms

CDC16, APC6, CDC16Hs, CDC16 homolog, Anaphase-promoting complex subunit 6

验证数据展示

  • WB analysis using 14307-1-AP

    WB analysis using 14307-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 14307-1-AP

    IHC staining of human cervical cancer using 14307-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cervical cancer using 14307-1-AP

    IHC staining of human cervical cancer using 14307-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 14307-1-AP

    IF Staining of HeLa using 14307-1-AP

    Immunofluorescent analysis of Hela cells, using CDC16 antibody 14307-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, NIH/3T3 cells, Jurakt cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14307-1-AP targets CDC16/APC6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CDC16/APC6 fusion protein Ag5427 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 16 homolog (S. cerevisiae)
Synonyms CDC16, APC6, CDC16Hs, CDC16 homolog, Anaphase-promoting complex subunit 6
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC010875
Gene Symbol CDC16/APC6
Gene ID (NCBI) 8881
RRIDAB_2074638
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13042
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDC16/APC6 antibody 14307-1-APDownload protocol
IHC protocol for CDC16/APC6 antibody 14307-1-APDownload protocol
IF protocol for CDC16/APC6 antibody 14307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols