• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CDC20 Recombinant antibody

CDC20 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241925E12

Cat no : 84531-5-RR

Print datasheet

Synonyms

p55CDC, CDC20A, 241925E12

验证数据展示

  • WB analysis using 84531-5-RR

    WB analysis using 84531-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84531-5-RR (CDC20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84531-5-RR

    Affinity and Kinetic Characterization of 84531-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84531-5-RR against Human CDC20 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, HepG2 cells, PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy CDC20 Ready-To-Use IHC Kit. CDC20 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84531-5-RR targets CDC20 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen CDC20 fusion protein Ag0295 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cell division cycle 20 homolog (S. cerevisiae)
Synonyms p55CDC, CDC20A, 241925E12
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC001088
Gene Symbol CDC20
Gene ID (NCBI) 991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ12834
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

实验方案

Product Specific Protocols
WB protocol for CDC20 antibody 84531-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols