CDC25B Polyclonal antibody

CDC25B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10644-1-AP

Print datasheet

Synonyms

CDC25B, CDC25HU2, M phase inducer phosphatase 2



经过测试的应用

Positive WB detected inmouse liver tissue, Jurkat cells, mouse brain tissue, Raji cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CDC25B Ready-To-Use IHC Kit. CDC25B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10644-1-AP targets CDC25B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen CDC25B fusion protein Ag0994 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 25 homolog B (S. pombe)
Synonyms CDC25B, CDC25HU2, M phase inducer phosphatase 2
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC006395
Gene Symbol CDC25B
Gene ID (NCBI) 994
RRIDAB_2075439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30305
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B).

实验方案

Product Specific Protocols
WB protocol for CDC25B antibody 10644-1-APDownload protocol
IHC protocol for CDC25B antibody 10644-1-APDownload protocol
IP protocol for CDC25B antibody 10644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB, IF

BMC Dev Biol

Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation.

Authors - Santanu De
mouseIF

BMC Dev Biol

YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation.

Authors - Alaa A Eisa