CDC25B Polyclonal antibody
CDC25B Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue, Jurkat cells, mouse brain tissue, Raji cells |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 2 publications below |
产品信息
10644-1-AP targets CDC25B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Immunogen | CDC25B fusion protein Ag0994 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cell division cycle 25 homolog B (S. pombe) |
Synonyms | CDC25B, CDC25HU2, M phase inducer phosphatase 2 |
Calculated Molecular Weight | 65 kDa |
Observed Molecular Weight | 60-70 kDa |
GenBank Accession Number | BC006395 |
Gene Symbol | CDC25B |
Gene ID (NCBI) | 994 |
RRID | AB_2075439 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P30305 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CDC25B antibody 10644-1-AP | Download protocol |
IHC protocol for CDC25B antibody 10644-1-AP | Download protocol |
IP protocol for CDC25B antibody 10644-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
BMC Dev Biol Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation. | ||
BMC Dev Biol YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation. |