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CDC27; APC3 Polyclonal antibody
CDC27; APC3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, K-562 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10918-1-AP targets CDC27; APC3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | CDC27; APC3 fusion protein Ag1345 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cell division cycle 27 homolog (S. cerevisiae) |
Synonyms | CDC27, CDC27 homolog, APC3, APC 3, Anaphase-promoting complex subunit 3 |
Calculated Molecular Weight | 92 kDa |
Observed Molecular Weight | 92 kDa |
GenBank Accession Number | BC011656 |
Gene Symbol | CDC27 |
Gene ID (NCBI) | 996 |
RRID | AB_2075288 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P30260 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CDC27; APC3 antibody 10918-1-AP | Download protocol |
IHC protocol for CDC27; APC3 antibody 10918-1-AP | Download protocol |
IF protocol for CDC27; APC3 antibody 10918-1-AP | Download protocol |
IP protocol for CDC27; APC3 antibody 10918-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Oncol CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in Neuroblastoma.
| ||
Oncotarget mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27. | ||
Cell Death Dis EGFR-ERK induced activation of GRHL1 promotes cell cycle progression by up-regulating cell cycle related genes in lung cancer. | ||
Cell Commun Signal APC/C-regulated CPT1C promotes tumor progression by upregulating the energy supply and accelerating the G1/S transition | ||
Cell Death Differ ABL1-mediated phosphorylation promotes FOXM1-related tumorigenicity by Increasing FOXM1 stability |