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验证数据展示

  • IHC staining of mouse testis using 13761-1-AP

    IHC staining of mouse testis using 13761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13761-1-AP (CDK8/19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 13761-1-AP

    IHC staining of mouse testis using 13761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13761-1-AP (CDK8/19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Y79 using 13761-1-AP

    WB analysis of Y79 using 13761-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13761-1-AP (CDK8/19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CDK8/19 Polyclonal antibody

CDK8/19 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13761-1-AP

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Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CDC2 related protein kinase 6, CDC2L6, CDK11, CDK19, CDK8, Cyclin dependent kinase 11, Death preventing kinase, KIAA1028

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells, mouse brain tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13761-1-AP targets CDK8/19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CDK8/19 fusion protein Ag4731 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 2-like 6 (CDK8-like)
Synonyms CDC2 related protein kinase 6, CDC2L6, CDK11, CDK19, CDK8, Cyclin dependent kinase 11, Death preventing kinase, KIAA1028
Calculated Molecular Weight 502 aa, 57 kDa
Observed Molecular Weight 50-57 kDa
GenBank Accession NumberBC037289
Gene Symbol CDK19
Gene ID (NCBI) 23097
RRIDAB_2276048
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BWU1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDK8/19 antibody 13761-1-APDownload protocol
IHC protocol for CDK8/19 antibody 13761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Mol Life Sci

Hotair promotes the migration and proliferation in ovarian cancer by miR-222-3p/CDK19 axis.

Authors - Lili Fan
View Article