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Species Reactivity
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验证数据展示

  • WB analysis of human pancreas using 10964-2-AP (same clone as 10964-2-PBS)

    WB analysis of human pancreas using 10964-2-AP (same clone as 10964-2-PBS)

    human pancreas tissue were subjected to SDS PAGE followed by western blot with 10964-2-AP (CDC34 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10964-2-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 10964-2-AP (same clone as 10964-2-PBS)

    IHC staining of human prostate cancer using 10964-2-AP (same clone as 10964-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10964-2-AP (CDC34 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10964-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 10964-2-AP (same clone as 10964-2-PBS)

    IP experiment of mouse testis using 10964-2-AP (same clone as 10964-2-PBS)

    IP result of anti-CDC34 (IP:10964-2-AP, 4ug; Detection:10964-2-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 10964-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CDC34 Polyclonal antibody, PBS Only

CDC34 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10964-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

10964-2-PBS targets CDC34 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CDC34 fusion protein Ag1438 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cell division cycle 34 homolog (S. cerevisiae)
别名
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC009850
基因名称 CDC34
Gene ID (NCBI) 997
RRIDAB_2077925
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49427
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDC34 is also named as UBCH3, UBE2R1(Ubiquitin-conjugating enzyme E2 R1) and belongs to the ubiquitin-conjugating enzyme family. Cdc34 enzyme is itself a substrate for the type of polyubiquitin modification that is known to cause targeting of proteins for degradation. It is phosphorylated in vivo, providing yet another potential means for control of its activity. And the predominantly nuclear localization of this enzyme suggests that substrate molecules relevant to its cell cycle function are similarly located within the nucleus(PMID:8164658). Human CDC34 could substitute efficiently for yeast CDC34(PMID:8248134). It can form a homodimer(PMID:8383676). This antibody can also recognize CDC34B(UBC3B) which is highly homologous to UBC3 (CDC34) with only 47 amino acid differences.