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  • Featured Product
  • KD/KO Validated

CDC34 Polyclonal antibody, PBS Only

CDC34 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10964-2-PBS

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Synonyms

验证数据展示

  • WB analysis of human pancreas using 10964-2-AP (same clone as 10964-2-PBS)

    WB analysis of human pancreas using 10964-2-AP (same clone as 10964-2-PBS)

    human pancreas tissue were subjected to SDS PAGE followed by western blot with 10964-2-AP (CDC34 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10964-2-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 10964-2-AP (same clone as 10964-2-PBS)

    IHC staining of human prostate cancer using 10964-2-AP (same clone as 10964-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10964-2-AP (CDC34 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10964-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 10964-2-AP (same clone as 10964-2-PBS)

    IP experiment of mouse testis using 10964-2-AP (same clone as 10964-2-PBS)

    IP result of anti-CDC34 (IP:10964-2-AP, 4ug; Detection:10964-2-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 10964-2-PBS in a different storage buffer formulation.

产品信息

10964-2-PBS targets CDC34 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CDC34 fusion protein Ag1438 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 34 homolog (S. cerevisiae)
Synonyms
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC009850
Gene Symbol CDC34
Gene ID (NCBI) 997
RRIDAB_2077925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49427
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDC34 is also named as UBCH3, UBE2R1(Ubiquitin-conjugating enzyme E2 R1) and belongs to the ubiquitin-conjugating enzyme family. Cdc34 enzyme is itself a substrate for the type of polyubiquitin modification that is known to cause targeting of proteins for degradation. It is phosphorylated in vivo, providing yet another potential means for control of its activity. And the predominantly nuclear localization of this enzyme suggests that substrate molecules relevant to its cell cycle function are similarly located within the nucleus(PMID:8164658). Human CDC34 could substitute efficiently for yeast CDC34(PMID:8248134). It can form a homodimer(PMID:8383676). This antibody can also recognize CDC34B(UBC3B) which is highly homologous to UBC3 (CDC34) with only 47 amino acid differences.