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  • Featured Product
  • KD/KO Validated

CDC37 Polyclonal antibody, PBS Only

CDC37 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10218-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HEK-293 using 10218-1-AP (same clone as 10218-1-PBS)

    WB analysis of HEK-293 using 10218-1-AP (same clone as 10218-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 10218-1-AP (same clone as 10218-1-PBS)

    IF Staining of HeLa using 10218-1-AP (same clone as 10218-1-PBS)

    Immunofluorescent analysis of HeLa cells using 10218-1-AP (Cdc37 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10218-1-AP (same clone as 10218-1-PBS)

    IHC staining of human colon cancer using 10218-1-AP (same clone as 10218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10218-1-AP (CDC37 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10218-1-AP (same clone as 10218-1-PBS)

    IHC staining of human colon cancer using 10218-1-AP (same clone as 10218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10218-1-AP (CDC37 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 10218-1-AP (same clone as 10218-1-PBS)

    IP experiment of HEK-293 using 10218-1-AP (same clone as 10218-1-PBS)

    IP result of anti-CDC37 (IP:10218-1-AP, 4ug; Detection:10218-1-AP 1:1000) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 10218-1-AP (same clone as 10218-1-PBS)

    FC experiment of MCF-7 using 10218-1-AP (same clone as 10218-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CDC37 Polyclonal antibody (10218-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

产品信息

10218-1-PBS targets CDC37 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, monkey
Immunogen CDC37 fusion protein Ag0267 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 37 homolog (S. cerevisiae)
Synonyms
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC000083
Gene Symbol CDC37
Gene ID (NCBI) 11140
RRIDAB_2077735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16543
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cdc (cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear.