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验证数据展示

  • WB analysis using 84679-4-RR

    WB analysis using 84679-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84679-4-RR (CDC40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84679-4-RR

    Affinity and Kinetic Characterization of 84679-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84679-4-RR against Human CDC40 were performed. The affinity constant is below 1 pM.

CDC40 Recombinant antibody

CDC40 Recombinant Antibody for WB, ELISA
Cat No. 84679-4-RR

产品说明书

CloneNo. 242034D1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Pre mRNA processing factor 17, hPRP17, EHB3, EH binding protein 3, Cell division cycle 40 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HUVEC cells, HeLa cells, Jurkat cells, THP-1 cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84679-4-RR targets CDC40 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 CDC40 fusion protein Ag36151 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cell division cycle 40 homolog (S. cerevisiae)
别名 Pre mRNA processing factor 17, hPRP17, EHB3, EH binding protein 3, Cell division cycle 40 homolog
观测分子量65-67 kDa
GenBank蛋白编号BC126114
基因名称 CDC40
Gene ID (NCBI) 51362
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO60508
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC40 (cell division cycle 40), also known as EHB3. It is expected to be located in the nucleus, which is ubiquitinated in bone marrow and lymph node. The protein is required for pre-mRNA splicing as component of the activated spliceosome, and it plays an important role in embryonic brain development; this function does not require proline isomerization (PMID: 33220177; 33220177). The molecular weight of CDC40 is 65 kDa.

实验方案

Product Specific Protocols
WB protocol for CDC40 antibody 84679-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols