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CDC42 Monoclonal antibody

CDC42 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G5E8

Cat no : 67212-1-Ig

Print datasheet

Synonyms

CDC42, CDC42Hs, G25K, G25K GTP binding protein

验证数据展示

  • WB analysis using 67212-1-Ig

    WB analysis using 67212-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67212-1-Ig

    WB analysis using 67212-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse cerebellum using 67212-1-Ig

    WB analysis of mouse cerebellum using 67212-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, rat brain tissue, mouse cerebellum tissue, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy CDC42 Ready-To-Use IHC Kit. CDC42 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67212-1-Ig targets CDC42 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen CDC42 fusion protein Ag28550 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name cell division cycle 42 (GTP binding protein, 25kDa)
Synonyms CDC42, CDC42Hs, G25K, G25K GTP binding protein
Calculated Molecular Weight 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC002711
Gene Symbol CDC42
Gene ID (NCBI) 998
RRIDAB_2918484
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP60953
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking

实验方案

Product Specific Protocols
WB protocol for CDC42 antibody 67212-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols