验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse thymus tissue |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
15678-1-AP targets CDC45L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | CDC45L fusion protein Ag8150 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CDC45 cell division cycle 45-like (S. cerevisiae) |
| 别名 | UNQ374/PRO710, PORC-PI-1, PORC PI 1, CDC45L2, CDC45 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 60-66 kDa |
| GenBank蛋白编号 | BC010022 |
| 基因名称 | CDC45L |
| Gene ID (NCBI) | 8318 |
| RRID | AB_10642704 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75419 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDC45L(Cell division control protein 45 homolog), plays a role in the initiation of DNA replication, and genetically interacts with MCM family members and with ORC2 and physically form a complex including Mcm5p. It's the first gene missed in DiGeorge syndrome that suggest involve in cell proliferation. This is a rabbit polyclonal antibody raised against part of C-terminus of human CDC45L.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC45L antibody 15678-1-AP | Download protocol |
| IHC protocol for CDC45L antibody 15678-1-AP | Download protocol |
| IF protocol for CDC45L antibody 15678-1-AP | Download protocol |
| IP protocol for CDC45L antibody 15678-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res The CRL4DTL E3 ligase induces degradation of the DNA replication initiation factor TICRR/TRESLIN specifically during S phase.
| ||
Oncotarget The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis. | ||
Ann Transl Med Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis. | ||
Signal Transduct Target Ther LSD1 is required for euchromatic origin firing and replication timing. | ||
Cell Cycle IGF2BP2 promotes glycolysis and hepatocellular carcinoma stemness by stabilizing CDC45 mRNA via m6A modification
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