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验证数据展示

  • IP experiment of mouse brain using 12974-1-AP

    IP experiment of mouse brain using 12974-1-AP

    IP result of anti-CDC5L (IP:12974-1-AP, 4ug; Detection:12974-1-AP 1:500) with mouse brain tissue lysate 1600 ug.

  • WB analysis of human brain using 12974-1-AP

    WB analysis of human brain using 12974-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 12974-1-AP

    IHC staining of mouse testis using 12974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 12974-1-AP

    IF Staining of HepG2 using 12974-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC5L antibody (12974-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CDC5L Polyclonal antibody

CDC5L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12974-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

PCDC5RP, KIAA0432, Cell division cycle 5-like protein, CEF1, Cdc5-like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells, HepG2 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

12974-1-AP targets CDC5L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 CDC5L fusion protein Ag3578 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CDC5 cell division cycle 5-like (S. pombe)
别名 PCDC5RP, KIAA0432, Cell division cycle 5-like protein, CEF1, Cdc5-like protein
计算分子量 802 aa, 92 kDa
观测分子量 100 kDa
GenBank蛋白编号BC001568
基因名称 CDC5L
Gene ID (NCBI) 988
RRIDAB_2078510
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99459
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDC5L antibody 12974-1-APDownload protocol
IHC protocol for CDC5L antibody 12974-1-APDownload protocol
IF protocol for CDC5L antibody 12974-1-APDownload protocol
IP protocol for CDC5L antibody 12974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncotarget

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Authors - Zhichao Gu
View Article
WB

J Biol Chem

O-GlcNAcylation of Thr12 / Ser56 in short form O-GlcNAc transferase (sOGT) regulates its substrate selectivity.

Authors - Li Liu
View Article
humanWB

Cancer Med

Circ-PGAM1 promotes malignant progression of epithelial ovarian cancer through regulation of the miR-542-3p/CDC5L/PEAK1 pathway.

Authors - Chunmei Zhang
View Article