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CDC6 Monoclonal antibody, PBS Only

CDC6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6F12D2

Cat no : 66021-1-PBS

Print datasheet

Synonyms

CDC6-related protein, CDC6 related protein, Cdc18-related protein, CDC18L, Cdc18 related protein

验证数据展示

  • WB analysis using 66021-1-Ig (same clone as 66021-1-PBS)

    WB analysis using 66021-1-Ig (same clone as 66021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66021-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66021-1-Ig (same clone as 66021-1-PBS)

    IF Staining of HeLa using 66021-1-Ig (same clone as 66021-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CDC6 antibody (66021-1-Ig, Clone: 6F12D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66021-1-PBS in a different storage buffer formulation.

产品信息

66021-1-PBS targets CDC6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CDC6 fusion protein Ag17958 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cell division cycle 6 homolog (S. cerevisiae)
Synonyms CDC6-related protein, CDC6 related protein, Cdc18-related protein, CDC18L, Cdc18 related protein
Calculated Molecular Weight 560 aa, 63 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC025232
Gene Symbol CDC6
Gene ID (NCBI) 990
RRIDAB_2881493
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99741
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human CDC6.