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验证数据展示

  • WB analysis of SH-SY5Y using 17980-1-AP

    WB analysis of SH-SY5Y using 17980-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17980-1-AP (Cdc7 Kinase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17980-1-AP

    IF Staining of HepG2 using 17980-1-AP

    Immunofluorescent analysis of HepG2 cells, using CDC7 antibody 17980-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Featured Product
  • KD/KO验证

Cdc7 Kinase Polyclonal antibody

Cdc7 Kinase Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17980-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

EC:2.7.11.1, Cell division cycle 7-related protein kinase, CDC7-related kinase, CDC7L1, CDC7 related kinase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17980-1-AP targets Cdc7 Kinase in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Cdc7 Kinase fusion protein Ag12384 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cell division cycle 7 homolog (S. cerevisiae)
别名 EC:2.7.11.1, Cell division cycle 7-related protein kinase, CDC7-related kinase, CDC7L1, CDC7 related kinase
计算分子量 574 aa, 64 kDa
观测分子量 64 kDa
GenBank蛋白编号BC111044
基因名称 CDC7
Gene ID (NCBI) 8317
RRIDAB_2291604
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00311
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Cdc7 Kinase antibody 17980-1-APDownload protocol
IF protocol for Cdc7 Kinase antibody 17980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

EGFR-ERK induced activation of GRHL1 promotes cell cycle progression by up-regulating cell cycle related genes in lung cancer.

Authors - Yiming He
View Article
humanWB

Cell Death Discov

Identifying CDC7 as a synergistic target of chemotherapy in resistant small-cell lung cancer via CRISPR/Cas9 screening

Authors - Ling Deng
  • KD Validated
View Article