验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11625-1-AP targets CDCA4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | CDCA4 fusion protein Ag2214 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cell division cycle associated 4 |
| 别名 | CDCA4, HEPP, SEI 3/HEPP |
| 计算分子量 | 241 aa, 26 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC025263 |
| 基因名称 | CDCA4 |
| Gene ID (NCBI) | 55038 |
| RRID | AB_2260414 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BXL8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIP-Br proteins are a set of proteins with the potential to function in both transcriptional control and cell cycle regulation [PMID:18572021]. CECA4 is a member of TRIP-br family, based on its homologous sequence motifs including SERTA and a PHD-bromo-binding site which interacts with a series of bromodomain containing transcriptional cofactors[PMID:17141982]. When activated by E2F, CDCA4 consequently functions as a suppressor of E2F-responsive promoters to participate the regulation of cell proliferation through the E2F/pRb pathway [PMID:16098148].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDCA4 antibody 11625-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell The DLEU2/miR-15a/16-1 cluster controls B cell proliferation and its deletion leads to chronic lymphocytic leukemia.
| ||
Sci Rep Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin. | ||
Cancer Cell Int CDCA4 suppresses epithelial-mesenchymal transtion (EMT) and metastasis in Non-small cell lung cancer through modulating autophagy.
| ||
Thorac Cancer CDCA4 interacts with IGF2BP1 to regulate lung adenocarcinoma proliferation via the PI3K/AKT pathway
| ||
Gene ALKBH5 promotes the development of lung adenocarcinoma by regulating the polarization of M2 macrophages through CDCA4
| ||
Front Mol Biosci Construction of shared gene signature between rheumatoid arthritis and lung adenocarcinoma helps to predict the prognosis and tumor microenvironment of the LUAD patients |
