验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, K-562 cells, LNCaP cells, U2OS cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29316-1-AP targets CDCA5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | CDCA5 fusion protein Ag30966 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cell division cycle associated 5 |
别名 | Sororin, p35, Cell division cycle-associated protein 5 |
计算分子量 | 27 kDa |
观测分子量 | 27~35 kDa |
GenBank蛋白编号 | BC011000 |
基因名称 | CDCA5 |
Gene ID (NCBI) | 113130 |
RRID | AB_3086115 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96FF9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human cell division cycle associated 5 (CDCA5), also known as Sororin, is required for stable binding of cohesin to chromatid in the S and G2/M phases and degraded through anaphase-promoting complex-dependent ubiquitination in the G0/G1 phase (PMID: 30808873). Suppression of CDCA5 expression with siRNAs inhibited the growth of lung cancer cells. CDCA5 positivity is an independent prognostic factor for lung cancer patients (PMID: 20551060). The predicted molecular size of CDCA is 27 kd, with an electrophoretic mobility of ~35 kd on SDS-PAGE, possibly due to its isoelectric point (pI) of approximately 10 (PMID: 22580470).
实验方案
Product Specific Protocols | |
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WB protocol for CDCA5 antibody 29316-1-AP | Download protocol |
IHC protocol for CDCA5 antibody 29316-1-AP | Download protocol |
IF protocol for CDCA5 antibody 29316-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |