验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse kidney tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CDCA8 Ready-To-Use IHC Kit. CDCA8 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12465-1-AP targets CDCA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CDCA8 fusion protein Ag3136 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cell division cycle associated 8 |
| 别名 | hDasra B, Dasra-B, Dasra B, Cell division cycle-associated protein 8, Borealin |
| 计算分子量 | 280 aa, 31 kDa |
| 观测分子量 | 31-38 kDa |
| GenBank蛋白编号 | BC016944 |
| 基因名称 | CDCA8 |
| Gene ID (NCBI) | 55143 |
| RRID | AB_2078783 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q53HL2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDCA8 antibody 12465-1-AP | Download protocol |
| IHC protocol for CDCA8 antibody 12465-1-AP | Download protocol |
| IF protocol for CDCA8 antibody 12465-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Pharmacol A novel risk model of three SUMOylation genes based on RNA expression for potential prognosis and treatment sensitivity prediction in kidney cancer | ||
J Cell Mol Med Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma | ||
Cell Mol Life Sci Global phosphoproteomic analysis identified key kinases regulating male meiosis in mouse | ||
Funct Integr Genomics Let-7c-5p down-regulates immune-related CDCA8 to inhibit hepatocellular carcinoma | ||
Am J Transl Res Cell division cycle associated 8 promotes the growth and inhibits the apoptosis of endometrial cancer cells by regulating cell cycle and P53/Rb signaling pathway
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