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验证数据展示

  • WB analysis using 68510-1-Ig

    WB analysis using 68510-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68510-1-Ig (CDCA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 68510-1-Ig

    IF Staining of A431 using 68510-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDCA8 antibody (68510-1-Ig, Clone: 2A10C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IF Staining of A549 using 68510-1-Ig

    IF Staining of A549 using 68510-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CDCA8 antibody (68510-1-Ig, Clone: 2A10C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

CDCA8 Monoclonal antibody

CDCA8 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68510-1-Ig

产品说明书

CloneNo. 2A10C9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Dasra-B, Dasra B, Cell division cycle-associated protein 8, Borealin, BOR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-468 cells, A431 cells, HEK-293 cell, K-562 cells, Ramos cells, Daudi cells
Positive IF/ICC detected inA549 cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy CDCA8 Ready-To-Use IHC Kit. CDCA8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68510-1-Ig targets CDCA8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CDCA8 fusion protein Ag3136 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cell division cycle associated 8
别名 Dasra-B, Dasra B, Cell division cycle-associated protein 8, Borealin, BOR
计算分子量 280 aa, 31 kDa
观测分子量 31-38 kDa
GenBank蛋白编号BC016944
基因名称 CDCA8
Gene ID (NCBI) 55143
RRIDAB_3085219
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ53HL2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The predicted molecular weight of CDCA8 is about 31 kDa. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification.

实验方案

Product Specific Protocols
WB protocol for CDCA8 antibody 68510-1-IgDownload protocol
IF protocol for CDCA8 antibody 68510-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols