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CDCP1 Polyclonal antibody

CDCP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12754-1-AP

Print datasheet

Synonyms

Transmembrane and associated with src kinases, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Membrane glycoprotein gp140, CUB domain-containing protein 1

验证数据展示

  • WB analysis using 12754-1-AP

    WB analysis using 12754-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12754-1-AP (CDCP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 12754-1-AP

    IHC staining of human skin cancer using 12754-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12754-1-AP (CDCP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 12754-1-AP

    IHC staining of human skin cancer using 12754-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12754-1-AP (CDCP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, PC-3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12754-1-AP targets CDCP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CDCP1 fusion protein Ag3439 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CUB domain containing protein 1
Synonyms Transmembrane and associated with src kinases, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Membrane glycoprotein gp140, CUB domain-containing protein 1
Calculated Molecular Weight 836 aa, 93 kDa
Observed Molecular Weight 72 kDa, 130 kDa
GenBank Accession NumberBC021099
Gene Symbol CDCP1
Gene ID (NCBI) 64866
RRIDAB_2078799
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H5V8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDCP1, also called CD318, TRASK, and SIMA 135, is a 135-140 kDa transmembrane protein containing three CUB domains within the extracellular region and a hexalysine stretch within the cytoplasmic region. A soluble form with MW 80 kDa (p80) is cleaved at an unidentified site on the N-terminal side. CDCP1 is a novel marker for hematopoietic stem cells.

实验方案

Product Specific Protocols
WB protocol for CDCP1 antibody 12754-1-APDownload protocol
IHC protocol for CDCP1 antibody 12754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Theranostics

Radiation-induced small extracellular vesicles as "carriages" promote tumor antigen release and trigger antitumor immunity.

Authors - Wanzun Lin
View Article
humanWB

Aging (Albany NY)

The emerging role of the MiR-1272-ADAM9-CDCP1 signaling pathway in the progression of glioma.

Authors - Fei Geng
View Article