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Cadherin-17 Monoclonal antibody

Cadherin-17 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3B10A9

Cat no : 60351-1-Ig

Print datasheet

Synonyms

CDH17, HPT1, HPT 1, CDH16, Cadherin 17

验证数据展示

  • WB analysis of human colon using 60351-1-Ig

    WB analysis of human colon using 60351-1-Ig

    Untreated and PNGase F-treated lysates of human colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • IHC staining of human colon cancer using 60351-1-Ig

    IHC staining of human colon cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDH17 antibody included
  • IHC staining of human colon cancer using 60351-1-Ig

    IHC staining of human colon cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDH17 antibody included
  • IF Staining of human colon cancer using 60351-1-Ig

    IF Staining of human colon cancer using 60351-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human colon cancer using 60351-1-Ig

    IF Staining of human colon cancer using 60351-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman colon tissue, pig colon tissue
Positive IHC detected inhuman colon cancer tissue, human colon tissue, human pancreas cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CDH17 Ready-To-Use IHC Kit. CDH17 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

60351-1-Ig targets Cadherin-17 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, pig
Cited Reactivityhuman
Immunogen Cadherin-17 fusion protein Ag19488 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cadherin 17, LI cadherin (liver-intestine)
Synonyms CDH17, HPT1, HPT 1, CDH16, Cadherin 17
Calculated Molecular Weight 832 aa, 92 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC113464
Gene Symbol Cadherin-17
Gene ID (NCBI) 1015
RRIDAB_2881460
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ12864
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Cadherin-17 antibody 60351-1-IgDownload protocol
IHC protocol for Cadherin-17 antibody 60351-1-IgDownload protocol
IF protocol for Cadherin-17 antibody 60351-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Biochim Biophys Acta Gen Subj

Gastric cancer patient-derived organoids model for the therapeutic drug screening

Authors - Jiao Xu
View Article