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  • Featured Product
  • KD/KO Validated

CDH26 Polyclonal antibody

CDH26 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20057-1-AP

Print datasheet

Synonyms

Cadherin-like protein VR20, Cadherin-like protein 26

验证数据展示

  • WB analysis using 20057-1-AP

    WB analysis using 20057-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20057-1-AP (CDH26 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 20057-1-AP

    WB analysis using 20057-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20057-1-AP (CDH26 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse liver using 20057-1-AP

    IF Staining of mouse liver using 20057-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CDH26 antibody (20057-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 20057-1-AP

    IF Staining of MCF-7 using 20057-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CDH26 antibody (20057-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inA549 cells, T-47D cells, HEK-293 cells, MCF-7 cells
Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20057-1-AP targets CDH26 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cadherin-like 26
Synonyms Cadherin-like protein VR20, Cadherin-like protein 26
Calculated Molecular Weight 95 kDa
Observed Molecular Weight18-22 kDa, 95-100 kDa
GenBank Accession NumberNM_021810
Gene Symbol CDH26
Gene ID (NCBI) 60437
RRIDAB_2878637
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IXH8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDH26, also named VR20, is a member of cadherins which are calcium-dependent cell adhesion proteins. Cadherins preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. CDH26 produces 4 isoforms through alternative splicing, with molecular weights of 13, 18, 92, and 95 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for CDH26 antibody 20057-1-APDownload protocol
IF protocol for CDH26 antibody 20057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Cell Discov

Cadherin-26 (CDH26) regulates airway epithelial cell cytoskeletal structure and polarity.

Authors - Marrah E Lachowicz-Scroggins
  • KD Validated
View Article