Anti-Human CDK1 (POH-1)
CDK1 Monoclonal Antibody for WB, FC (Intra)
Host / Isotype
Mouse / IgG2a, kappa
Reactivity
Human, Bovine and More (1)
Applications
WB, FC (Intra)
Conjugate
Unconjugated
CloneNo.
POH-1
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells, K-562 cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CDK1 Ready-To-Use IHC Kit. CDK1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
65182-1-Ig targets CDK1 in WB, FC (Intra) applications and shows reactivity with Human, Bovine samples.
| Tested Applications | WB, FC (Intra) Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, Bovine |
| Cited Reactivity | human |
| Immunogen | Bacterially expressed full-length human Cdk1 protein 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cell division cycle 2, G1 to S and G2 to M |
| Synonyms | CDC2, CDC28A, CDK1, Cell division protein kinase 1, Cyclin dependent kinase 1, DKFZp686L20222, p34 protein kinase |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 30-34 kDa |
| GenBank Accession Number | BC014563 |
| Gene Symbol | CDK1 |
| Gene ID (NCBI) | 983 |
| RRID | AB_2918456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Purified by protein-A affinity chromatography |
| UNIPROT ID | P06493 |
| Storage Buffer | PBS with 0.1% sodium azide. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK1 antibody 65182-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Genomics Proteomics Depletion of DNTTIP2 Induces Cell Cycle Arrest in Pancreatic Cancer Cells |
