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Conjugate
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Conjugate
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Conjugate
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CDK1 Monoclonal antibody, PBS Only

CDK1 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2A11E4

Cat no : 67575-1-PBS

Print datasheet

Synonyms

CDC2, 2A11E4, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1

验证数据展示

  • WB analysis using 67575-1-Ig (same clone as 67575-1-PBS)

    WB analysis using 67575-1-Ig (same clone as 67575-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67575-1-Ig (CDK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67575-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67575-1-Ig (same clone as 67575-1-PBS)

    FC experiment of HeLa using 67575-1-Ig (same clone as 67575-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK1 Monoclonal antibody (67575-1-Ig, Clone:2A11E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67575-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CDK1 Ready-To-Use IHC Kit. CDK1 primary antibody included.

产品信息

67575-1-PBS targets CDK1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CDK1 fusion protein Ag29018 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name cell division cycle 2, G1 to S and G2 to M
Synonyms CDC2, 2A11E4, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC014563
Gene Symbol CDK1
Gene ID (NCBI) 983
RRIDAB_2882788
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06493
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.