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CDK1 Recombinant antibody

CDK1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240937C5

Cat no : 84271-1-RR

Print datasheet

Synonyms

CDC2, 240937C5, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1

验证数据展示

  • WB analysis using 84271-1-RR

    WB analysis using 84271-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84271-1-RR (CDK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84271-1-RR

    Affinity and Kinetic Characterization of 84271-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84271-1-RR against Human CDK1 were performed. The affinity constant is 34.3 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, C6 cells
Planning an IHC experiment? We recommend our IHCeasy CDK1 Ready-To-Use IHC Kit. CDK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84271-1-RR targets CDK1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CDK1 fusion protein Ag29018 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cell division cycle 2, G1 to S and G2 to M
Synonyms CDC2, 240937C5, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC014563
Gene Symbol CDK1
Gene ID (NCBI) 983
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP06493
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa. This antibody may have cross reaction with CDK2.

实验方案

Product Specific Protocols
WB protocol for CDK1 antibody 84271-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols