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CDK2 Monoclonal antibody, PBS Only

CDK2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4E2E10

Cat no : 60312-1-PBS

Print datasheet

Synonyms

EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2

验证数据展示

  • WB analysis of K-562 using 60312-1-Ig (same clone as 60312-1-PBS)

    WB analysis of K-562 using 60312-1-Ig (same clone as 60312-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60312-1-Ig (CDK2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60312-1-Ig (CDK2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK2 antibody included
  • IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60312-1-Ig (CDK2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK2 antibody included
  • IF Staining of HeLa using 60312-1-Ig (same clone as 60312-1-PBS)

    IF Staining of HeLa using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CDK2 antibody (60312-1-Ig, Clone: 4E2E10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 60312-1-Ig (same clone as 60312-1-PBS)

    FC experiment of HepG2 using 60312-1-Ig (same clone as 60312-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CDK2 Monoclonal antibody (60312-1-Ig, Clone:4E2E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CDK2 Ready-To-Use IHC Kit. CDK2 primary antibody included.

产品信息

60312-1-PBS targets CDK2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen CDK2 fusion protein Ag21310 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name cyclin-dependent kinase 2
Synonyms EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC003065
Gene Symbol CDK2
Gene ID (NCBI) 1017
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP24941
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.