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CDK2 Monoclonal antibody, PBS Only

CDK2 Monoclonal Antibody for WB,IHC,IF,FC,ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB,IHC,IF,FC,ELISA

Conjugate

Unconjugated

CloneNo.

4E2E10

Cat No : 60312-1-PBS

Print datasheet

Synonyms

CDK2, Cell division protein kinase 2, cyclin dependent kinase 2, p33 protein kinase, p33(CDK2)

验证数据展示

  • WB analysis of K-562 using 60312-1-Ig (same clone as 60312-1-PBS)

    WB analysis of K-562 using 60312-1-Ig (same clone as 60312-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60312-1-Ig (CDK2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60312-1-Ig (CDK2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60312-1-Ig (CDK2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60312-1-Ig (same clone as 60312-1-PBS)

    IF Staining of HeLa using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CDK2 antibody (60312-1-Ig, Clone: 4E2E10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 60312-1-Ig (same clone as 60312-1-PBS)

    FC experiment of Jurkat using 60312-1-Ig (same clone as 60312-1-PBS)

    1X10^6 Jurkat cells were stained with 0.2ug CDK2 antibody (60312-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

产品信息

60312-1-PBS targets CDK2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB,IHC,IF,FC,ELISA
Tested Reactivity human
Immunogen CDK2 fusion protein Ag21310 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name cyclin-dependent kinase 2
Synonyms CDK2, Cell division protein kinase 2, cyclin dependent kinase 2, p33 protein kinase, p33(CDK2)
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC003065
Gene Symbol CDK2
Gene ID (NCBI) 1017
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP24941
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.