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验证数据展示

  • WB analysis using 83635-2-RR (same clone as 83635-2-PBS)

    WB analysis using 83635-2-RR (same clone as 83635-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83635-2-RR (CDK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83635-2-RR (same clone as 83635-2-PBS)

    IHC staining of human ovarian cancer using 83635-2-RR (same clone as 83635-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83635-2-RR (CDK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK2 antibody included
  • IHC staining of human ovarian cancer using 83635-2-RR (same clone as 83635-2-PBS)

    IHC staining of human ovarian cancer using 83635-2-RR (same clone as 83635-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83635-2-RR (CDK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK2 antibody included
  • IF Staining of HepG2 using 83635-2-RR (same clone as 83635-2-PBS)

    IF Staining of HepG2 using 83635-2-RR (same clone as 83635-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK2 antibody (83635-2-RR, Clone: 240580G11 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83635-2-RR (same clone as 83635-2-PBS)

    FC experiment of HeLa using 83635-2-RR (same clone as 83635-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK2 Recombinant antibody (83635-2-RR, Clone:240580G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00611-1

    Cytometric bead array standard curve of MP00611-1

    Cytometric bead array standard curve of MP00611-1, CDK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83635-3-PBS. Detection antibody: 83635-2-PBS. Standard: Ag17133. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP00611-2

    Cytometric bead array standard curve of MP00611-2

    Cytometric bead array standard curve of MP00611-2, CDK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83635-2-PBS. Detection antibody: 83635-1-PBS. Standard: Ag17133. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 83635-2-PBS

    Affinity and Kinetic Characterization of 83635-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83635-2-PBS against Human CDK2 was performed. The affinity constant is 4.09 nM.

  • Affinity and Kinetic Characterization of 83635-2-RR

    Affinity and Kinetic Characterization of 83635-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83635-2-RR against Human CDK2 were performed. The affinity constant is 3.23 nM.

CDK2 Recombinant antibody, PBS Only (Capture/Detector)

CDK2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83635-2-PBS

产品说明书

CloneNo. 240580G11

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CDK2 Ready-To-Use IHC Kit. CDK2 primary antibody included.

产品信息

83635-2-PBS targets CDK2 as part of a matched antibody pair:

MP00611-1: 83635-3-PBS capture and 83635-2-PBS detection (validated in Cytometric bead array)

MP00611-2: 83635-2-PBS capture and 83635-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 CDK2 fusion protein Ag17133 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 2
别名 EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2
计算分子量 298 aa, 34 kDa
观测分子量 30-33 kDa
GenBank蛋白编号BC003065
基因名称 CDK2
Gene ID (NCBI) 1017
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24941
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.