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验证数据展示

  • WB analysis using 83635-4-RR

    WB analysis using 83635-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83635-4-RR (CDK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83635-4-RR

    FC experiment of HeLa using 83635-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK2 Recombinant antibody (83635-4-RR, Clone:240580A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83635-4-RR

    Affinity and Kinetic Characterization of 83635-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83635-4-RR against Human CDK2 were performed. The affinity constant is 8.32 nM.

CDK2 Recombinant antibody

CDK2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83635-4-RR

产品说明书

CloneNo. 240580A11
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, FC (Intra), ELISA

别名

EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, Jurkat cells, K-562 cells, HEK-293 cells, HeLa cells, HepG2 cells, C6 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDK2 Ready-To-Use IHC Kit. CDK2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

83635-4-RR targets CDK2 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性human
免疫原 CDK2 fusion protein Ag17133 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 2
别名 EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2
计算分子量 298 aa, 34 kDa
观测分子量 30-33 kDa
GenBank蛋白编号BC003065
基因名称 CDK2
Gene ID (NCBI) 1017
RRIDAB_3671246
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP24941
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for CDK2 antibody 83635-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Discov Oncol

Unveiling the role of PSMA5 in glioma progression and prognosis

Authors - Wei Liu
View Article