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验证数据展示

  • WB analysis using 83635-5-RR (same clone as 83635-5-PBS)

    WB analysis using 83635-5-RR (same clone as 83635-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83635-5-RR (CDK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83635-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83635-5-RR (same clone as 83635-5-PBS)

    IF Staining of HeLa using 83635-5-RR (same clone as 83635-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2 antibody (83635-5-RR, Clone: 240580A6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83635-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83635-5-RR (same clone as 83635-5-PBS)

    IF Staining of HepG2 using 83635-5-RR (same clone as 83635-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK2 antibody (83635-5-RR, Clone: 240580A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83635-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83635-5-RR (same clone as 83635-5-PBS)

    FC experiment of HeLa using 83635-5-RR (same clone as 83635-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK2 Recombinant antibody (83635-5-RR, Clone:240580A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83635-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83635-5-RR

    Affinity and Kinetic Characterization of 83635-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83635-5-RR against Human CDK2 were performed. The affinity constant is 3.84 nM.

CDK2 Recombinant antibody, PBS Only

CDK2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83635-5-PBS

产品说明书

CloneNo. 240580A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CDK2 Ready-To-Use IHC Kit. CDK2 primary antibody included.

产品信息

83635-5-PBS targets CDK2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 CDK2 fusion protein Ag17133 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 2
别名 EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2
计算分子量 298 aa, 34 kDa
观测分子量 30 kDa
GenBank蛋白编号BC003065
基因名称 CDK2
Gene ID (NCBI) 1017
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP24941
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily. It is dispensable for myelination but is important for adult oligodendrocyte progenitor cell renewal, and could be one of the underlying mechanisms that drive adult progenitors to differentiate and thus regenerate myelin(PMID:21502361). G2 phase CCNA1/CDK2 controls the timing of entry into mitosis by controlling the subsequent activation of CCNB/CDK1, but also has an unexpected role in coordinating the activation of CCNB/CDK1 at the centrosome and in the nucleus(PMID:18372919).