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CDK2AP1 Polyclonal antibody

CDK2AP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13060-2-AP

Print datasheet

Synonyms

DOC 1, Deleted in oral cancer 1, Cyclin-dependent kinase 2-associated protein 1, CDKAP1, CDK2-associated protein 1

验证数据展示

  • WB analysis of HEK-293 using 13060-2-AP

    WB analysis of HEK-293 using 13060-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 13060-2-AP

    IF Staining of HeLa using 13060-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2AP1 antibody (13060-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human testis using 13060-2-AP

    IHC staining of human testis using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CDK2AP1 antibody included
  • IHC staining of human testis using 13060-2-AP

    IHC staining of human testis using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CDK2AP1 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human liver tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDK2AP1 Ready-To-Use IHC Kit. CDK2AP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13060-2-AP targets CDK2AP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen CDK2AP1 fusion protein Ag3736 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase 2 associated protein 1
Synonyms DOC 1, Deleted in oral cancer 1, Cyclin-dependent kinase 2-associated protein 1, CDKAP1, CDK2-associated protein 1
Calculated Molecular Weight 115 aa, 13 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC034717
Gene Symbol CDK2AP1
Gene ID (NCBI) 8099
RRIDAB_10340136
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14519
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDK2AP1 antibody 13060-2-APDownload protocol
IHC protocol for CDK2AP1 antibody 13060-2-APDownload protocol
IF protocol for CDK2AP1 antibody 13060-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncol Lett

Cathepsin L interacts with CDK2-AP1 as a potential predictor of prognosis in patients with breast cancer.

Authors - Zhan Wang
View Article