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验证数据展示

  • WB analysis of HEK-293 using 13060-2-AP

    WB analysis of HEK-293 using 13060-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 13060-2-AP

    IF Staining of HeLa using 13060-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2AP1 antibody (13060-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human testis using 13060-2-AP

    IHC staining of human testis using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CDK2AP1 antibody included
  • IHC staining of human testis using 13060-2-AP

    IHC staining of human testis using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CDK2AP1 antibody included

CDK2AP1 Polyclonal antibody

CDK2AP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13060-2-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

DOC 1, Deleted in oral cancer 1, Cyclin-dependent kinase 2-associated protein 1, CDKAP1, CDK2-associated protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human liver tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDK2AP1 Ready-To-Use IHC Kit. CDK2AP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13060-2-AP targets CDK2AP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 CDK2AP1 fusion protein Ag3736 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase 2 associated protein 1
别名 DOC 1, Deleted in oral cancer 1, Cyclin-dependent kinase 2-associated protein 1, CDKAP1, CDK2-associated protein 1
计算分子量 115 aa, 13 kDa
观测分子量 12 kDa
GenBank蛋白编号BC034717
基因名称 CDK2AP1
Gene ID (NCBI) 8099
RRIDAB_10340136
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14519
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDK2AP1 antibody 13060-2-APDownload protocol
IHC protocol for CDK2AP1 antibody 13060-2-APDownload protocol
IF protocol for CDK2AP1 antibody 13060-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncol Lett

Cathepsin L interacts with CDK2-AP1 as a potential predictor of prognosis in patients with breast cancer.

Authors - Zhan Wang
View Article