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CDK2AP1 Polyclonal antibody

CDK2AP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13060-2-AP

Print datasheet

Synonyms

DOC 1, Deleted in oral cancer 1, Cyclin-dependent kinase 2-associated protein 1, CDKAP1, CDK2-associated protein 1

验证数据展示

  • WB analysis of HEK-293 using 13060-2-AP

    WB analysis of HEK-293 using 13060-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 13060-2-AP

    IF Staining of HeLa using 13060-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2AP1 antibody (13060-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDK2AP1 Ready-To-Use IHC Kit. CDK2AP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13060-2-AP targets CDK2AP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen CDK2AP1 fusion protein Ag3736 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase 2 associated protein 1
Synonyms DOC 1, Deleted in oral cancer 1, Cyclin-dependent kinase 2-associated protein 1, CDKAP1, CDK2-associated protein 1
Calculated Molecular Weight 115 aa, 13 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC034717
Gene Symbol CDK2AP1
Gene ID (NCBI) 8099
RRIDAB_10340136
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14519
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDK2AP1 antibody 13060-2-APDownload protocol
IHC protocol for CDK2AP1 antibody 13060-2-APDownload protocol
IF protocol for CDK2AP1 antibody 13060-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncol Lett

Cathepsin L interacts with CDK2-AP1 as a potential predictor of prognosis in patients with breast cancer.

Authors - Zhan Wang
View Article