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CDK4 Monoclonal antibody, PBS Only

CDK4 Monoclonal Antibody for WB,IHC,FC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,IHC,FC,ELISA

Conjugate

Unconjugated

CloneNo.

1G2C12

Cat No : 66950-1-PBS

Print datasheet

Synonyms

CDK4, Cell division protein kinase 4, CMM3, cyclin dependent kinase 4, PSK J3

验证数据展示

  • WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • WB analysis of 4T1 using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis of 4T1 using 66950-1-Ig (same clone as 66950-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 66950-1-Ig (same clone as 66950-1-PBS)

    FC experiment of MCF-7 using 66950-1-Ig (same clone as 66950-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human CDK4 (66950-1-Ig, Clone:1G2C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66950-1-Ig (CDK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK4 antibody included
  • IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66950-1-Ig (CDK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK4 antibody included
  • WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CDK4 Ready-To-Use IHC Kit. CDK4 primary antibody included.

产品信息

66950-1-PBS targets CDK4 in WB, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,IHC,FC,ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen CDK4 fusion protein Ag20538 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cyclin-dependent kinase 4
Synonyms CDK4, Cell division protein kinase 4, CMM3, cyclin dependent kinase 4, PSK J3
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC010153
Gene Symbol CDK4
Gene ID (NCBI) 1019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11802
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).