• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • WB analysis of 4T1 using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis of 4T1 using 66950-1-Ig (same clone as 66950-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 66950-1-Ig (same clone as 66950-1-PBS)

    FC experiment of MCF-7 using 66950-1-Ig (same clone as 66950-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human CDK4 (66950-1-Ig, Clone:1G2C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66950-1-Ig (CDK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK4 antibody included
  • IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66950-1-Ig (CDK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK4 antibody included
  • WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

CDK4 Monoclonal antibody, PBS Only

CDK4 Monoclonal Antibody for ELISA, IHC, WB
Cat No. 66950-1-PBS

产品说明书

CloneNo. 1G2C12

宿主/亚型

Mouse / IgG1

种属反应性

rat, mouse, human

应用

ELISA, IHC, WB

别名

PSK J3, EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy CDK4 Ready-To-Use IHC Kit. CDK4 primary antibody included.

产品信息

66950-1-PBS targets CDK4 in ELISA, IHC, WB applications and shows reactivity with rat, mouse, human samples.

经测试应用 ELISA, IHC, WB Application Description
经测试反应性 rat, mouse, human
免疫原 CDK4 fusion protein Ag20538 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin-dependent kinase 4
别名 PSK J3, EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC010153
基因名称 CDK4
Gene ID (NCBI) 1019
RRIDAB_2882273
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11802
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).