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CDK4 Recombinant antibody

CDK4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

241053F10

Cat no : 83994-3-RR

Print datasheet

Synonyms

EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3, Cell division protein kinase 4

验证数据展示

  • WB analysis using 83994-3-RR

    WB analysis using 83994-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83994-3-RR (CDK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 83994-3-RR

    IP experiment of HeLa using 83994-3-RR

    IP result of anti-CDK4 (IP:83994-3-RR, 4ug; Detection:83994-3-RR 1:500) with HeLa cells lysate 1280 ug.

  • IF Staining of MCF-7 using 83994-3-RR

    IF Staining of MCF-7 using 83994-3-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CDK4 antibody (83994-3-RR, Clone: 241053F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human cervical squamous cancer using 83994-3-RR

    IHC staining of human cervical squamous cancer using 83994-3-RR

    Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83994-3-RR (CDK4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK4 antibody included

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, MCF-7 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical squamous cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CDK4 Ready-To-Use IHC Kit. CDK4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83994-3-RR is CDK4 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen CDK4 fusion protein Ag20538 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cyclin-dependent kinase 4
Synonyms EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3, Cell division protein kinase 4
Calculated Molecular Weight 34 kDa
Observed Molecular Weight30-34 kDa
GenBank Accession NumberBC010153
Gene Symbol CDK4
Gene ID (NCBI) 1019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP11802
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1-to S-phase transition during the cell cycle, and its expression is primarily controlled at the transcriptional level(PMID:17253961). The CCND1-CDK4 axis is critical not only in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment, which are vital to sustain tumor outgrowth (PMID:21844184).

实验方案

Product Specific Protocols
WB protocol for CDK4 antibody 83994-3-RRDownload protocol
IHC protocol for CDK4 antibody 83994-3-RRDownload protocol
IF protocol for CDK4 antibody 83994-3-RRDownload protocol
IP protocol for CDK4 antibody 83994-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols