• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83994-3-RR

    WB analysis using 83994-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83994-3-RR (CDK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 83994-3-RR

    IP experiment of HeLa using 83994-3-RR

    IP result of anti-CDK4 (IP:83994-3-RR, 4ug; Detection:83994-3-RR 1:500) with HeLa cells lysate 1280 ug.

  • IF Staining of MCF-7 using 83994-3-RR

    IF Staining of MCF-7 using 83994-3-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CDK4 antibody (83994-3-RR, Clone: 241053F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human cervical squamous cancer using 83994-3-RR

    IHC staining of human cervical squamous cancer using 83994-3-RR

    Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83994-3-RR (CDK4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK4 antibody included

CDK4 Recombinant antibody

CDK4 Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 83994-3-RR

产品说明书

CloneNo. 241053F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3, Cell division protein kinase 4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, MCF-7 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical squamous cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CDK4 Ready-To-Use IHC Kit. CDK4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83994-3-RR targets CDK4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 CDK4 fusion protein Ag20538 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 4
别名 EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3, Cell division protein kinase 4
计算分子量 34 kDa
观测分子量30-34 kDa
GenBank蛋白编号BC010153
基因名称 CDK4
Gene ID (NCBI) 1019
RRIDAB_3671570
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP11802
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1-to S-phase transition during the cell cycle, and its expression is primarily controlled at the transcriptional level(PMID:17253961). The CCND1-CDK4 axis is critical not only in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment, which are vital to sustain tumor outgrowth (PMID:21844184).

实验方案

Product Specific Protocols
WB protocol for CDK4 antibody 83994-3-RRDownload protocol
IHC protocol for CDK4 antibody 83994-3-RRDownload protocol
IF protocol for CDK4 antibody 83994-3-RRDownload protocol
IP protocol for CDK4 antibody 83994-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols