验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HCT 116 cells, mouse pancreas tissue, NIH/3T3 cells, WB result of CDK5 antibody (10430-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and CDK5 knockout HCT 116 cells., mouse brain tissue |
| Positive IHC detected in | mouse kidney tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy CDK5 Ready-To-Use IHC Kit. CDK5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10430-1-AP targets CDK5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CDK5 fusion protein Ag0686 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cyclin-dependent kinase 5 |
| 别名 | Serine/threonine-protein kinase PSSALRE, PSSALRE, EC:2.7.11.1, Cyclin-dependent-like kinase 5, Cyclin-dependent kinase 5 |
| 计算分子量 | 31 kDa, 33 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC005115 |
| 基因名称 | CDK5 |
| Gene ID (NCBI) | 1020 |
| RRID | AB_2078859 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q00535 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK5 antibody 10430-1-AP | Download protocol |
| IHC protocol for CDK5 antibody 10430-1-AP | Download protocol |
| IF protocol for CDK5 antibody 10430-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis | ||
Neuropharmacology Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex.
| ||
Cells Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis.
| ||
Am J Transl Res Hypoxia induces HT-22 neuronal cell death via Orai1/CDK5 pathway-mediated Tau hyperphosphorylation. | ||
J Cell Biochem Nestin protects mouse podocytes against high glucose-induced apoptosis by a Cdk5-dependent mechanism. | ||
BMC Neurol The LPA-CDK5-tau pathway mediates neuronal injury in an in vitro model of ischemia-reperfusion insult. |
