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- KD/KO Validated
CDK5 Polyclonal antibody
CDK5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, mouse pancreas tissue, NIH/3T3 cells, mouse brain tissue |
Positive IHC detected in | mouse kidney tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 13 publications below |
产品信息
10430-1-AP targets CDK5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | CDK5 fusion protein Ag0686 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cyclin-dependent kinase 5 |
Synonyms | Serine/threonine-protein kinase PSSALRE, PSSALRE, EC:2.7.11.1, Cyclin-dependent-like kinase 5, Cyclin-dependent kinase 5 |
Calculated Molecular Weight | 31 kDa, 33 kDa |
Observed Molecular Weight | 33 kDa |
GenBank Accession Number | BC005115 |
Gene Symbol | CDK5 |
Gene ID (NCBI) | 1020 |
RRID | AB_2078859 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q00535 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CDK5 antibody 10430-1-AP | Download protocol |
IHC protocol for CDK5 antibody 10430-1-AP | Download protocol |
IF protocol for CDK5 antibody 10430-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis | ||
Neuropharmacology Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex.
| ||
Cells Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis.
| ||
Am J Transl Res Hypoxia induces HT-22 neuronal cell death via Orai1/CDK5 pathway-mediated Tau hyperphosphorylation. | ||
J Cell Biochem Nestin protects mouse podocytes against high glucose-induced apoptosis by a Cdk5-dependent mechanism. | ||
BMC Neurol The LPA-CDK5-tau pathway mediates neuronal injury in an in vitro model of ischemia-reperfusion insult. |