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CDK5 Monoclonal antibody

CDK5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B9E1

Cat no : 68514-1-Ig

Print datasheet

Synonyms

CDK5, Cell division protein kinase 5, cyclin dependent kinase 5, PSSALRE, TPKII catalytic subunit

验证数据展示

  • WB analysis using 68514-1-Ig

    WB analysis using 68514-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68514-1-Ig (CDK5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68514-1-Ig

    IHC staining of mouse brain using 68514-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68514-1-Ig (CDK5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK5 antibody included
  • IHC staining of mouse brain using 68514-1-Ig

    IHC staining of mouse brain using 68514-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68514-1-Ig (CDK5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK5 antibody included
  • IF Staining of SH-SY5Y using 68514-1-Ig

    IF Staining of SH-SY5Y using 68514-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CDK5 antibody (68514-1-Ig, Clone: 1B9E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells, PC-3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy CDK5 Ready-To-Use IHC Kit. CDK5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68514-1-Ig targets CDK5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen CDK5 fusion protein Ag28465 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cyclin-dependent kinase 5
Synonyms CDK5, Cell division protein kinase 5, cyclin dependent kinase 5, PSSALRE, TPKII catalytic subunit
Calculated Molecular Weight 31 kDa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC005115
Gene Symbol CDK5
Gene ID (NCBI) 1020
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00535
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.

实验方案

Product Specific Protocols
WB protocol for CDK5 antibody 68514-1-IgDownload protocol
IHC protocol for CDK5 antibody 68514-1-IgDownload protocol
IF protocol for CDK5 antibody 68514-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols