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验证数据展示

  • WB analysis using 68514-1-Ig

    WB analysis using 68514-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68514-1-Ig (CDK5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68514-1-Ig

    IHC staining of mouse brain using 68514-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68514-1-Ig (CDK5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK5 antibody included
  • IHC staining of mouse brain using 68514-1-Ig

    IHC staining of mouse brain using 68514-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68514-1-Ig (CDK5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK5 antibody included
  • IF Staining of SH-SY5Y using 68514-1-Ig

    IF Staining of SH-SY5Y using 68514-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CDK5 antibody (68514-1-Ig, Clone: 1B9E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CDK5 Monoclonal antibody

CDK5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68514-1-Ig

产品说明书

CloneNo. 1B9E1

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CDK5, Cell division protein kinase 5, cyclin dependent kinase 5, PSSALRE, TPKII catalytic subunit

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells, PC-3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy CDK5 Ready-To-Use IHC Kit. CDK5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68514-1-Ig targets CDK5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 CDK5 fusion protein Ag28465 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin-dependent kinase 5
别名 CDK5, Cell division protein kinase 5, cyclin dependent kinase 5, PSSALRE, TPKII catalytic subunit
计算分子量 31 kDa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC005115
基因名称 CDK5
Gene ID (NCBI) 1020
RRIDAB_3085223
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ00535
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.

实验方案

Product Specific Protocols
WB protocol for CDK5 antibody 68514-1-IgDownload protocol
IHC protocol for CDK5 antibody 68514-1-IgDownload protocol
IF protocol for CDK5 antibody 68514-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols