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CDK5R1 Monoclonal antibody, PBS Only

CDK5R1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A3A5

Cat no : 67503-1-PBS

Print datasheet

Synonyms

CDK5 activator 1, CDK5P35, CDK5R, CDK5R1, NCK5A, p23, p25, p35, p35nck5a, TPKII regulatory subunit

验证数据展示

  • WB analysis using 67503-1-Ig (same clone as 67503-1-PBS)

    WB analysis using 67503-1-Ig (same clone as 67503-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

    IHC staining of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK5R1 antibody included
  • IHC staining of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

    IHC staining of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK5R1 antibody included
  • WB analysis of mouse cerebellum using 67503-1-Ig (same clone as 67503-1-PBS)

    WB analysis of mouse cerebellum using 67503-1-Ig (same clone as 67503-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CDK5R1 Ready-To-Use IHC Kit. CDK5R1 primary antibody included.

产品信息

67503-1-PBS targets CDK5R1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen CDK5R1 fusion protein Ag13152 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cyclin-dependent kinase 5, regulatory subunit 1 (p35)
Synonyms CDK5 activator 1, CDK5P35, CDK5R, CDK5R1, NCK5A, p23, p25, p35, p35nck5a, TPKII regulatory subunit
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC020580
Gene Symbol CDK5R1
Gene ID (NCBI) 8851
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15078
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin dependent kinase-5 (Cdk5), is an atypical but essential member of the Cdk family of proline-directed serine/threonine kinases. CDK5R1 encodes p35, a specific activator of the serine/threonine kinase CDK5, which plays a central role in neuronal migration and differentiation during central nervous system development. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form.