• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CDK5R2/p39 Polyclonal antibody

CDK5R2/p39 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27058-1-AP

Print datasheet

Synonyms

CDK5 activator 2, CDK5R2, CDK5R2/p39, NCK5AI, P39, p39I, p39nck5ai

验证数据展示

  • WB analysis of Neuro-2a using 27058-1-AP

    WB analysis of Neuro-2a using 27058-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27058-1-AP (CDK5R2/p39 antibody at dilution of 1:600 incubated at 4 degree celsius over night.

  • WB analysis using 27058-1-AP

    WB analysis using 27058-1-AP

    various lysates were subjected to SDS PAGE followed by western blot with 27058-1-AP (CDK5R2/p39 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 27058-1-AP

    IHC staining of mouse brain using 27058-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27058-1-AP (CDK5R2/p39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNeuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27058-1-AP targets CDK5R2/p39 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CDK5R2/p39 fusion protein Ag25834 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase 5, regulatory subunit 2 (p39)
Synonyms CDK5 activator 2, CDK5R2, CDK5R2/p39, NCK5AI, P39, p39I, p39nck5ai
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC041771
Gene Symbol CDK5R2/p39
Gene ID (NCBI) 8941
RRIDAB_2880735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13319
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK5R2 is a neuron-specific activator of CDK5 kinase. It associates with CDK5 to form an active kinase. This protein and neuron-specific CDK5 activator CDK5R1/p39NCK5A both share limited similarity to cyclins, and thus may define a distinct family of cyclin-dependent kinase activating proteins.

实验方案

Product Specific Protocols
WB protocol for CDK5R2/p39 antibody 27058-1-APDownload protocol
IHC protocol for CDK5R2/p39 antibody 27058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

BMC Cancer

m1A regulator-mediated methylation modification patterns correlated with autophagy to predict the prognosis of hepatocellular carcinoma

Authors - Yingmin Wu
View Article