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  • Featured Product
  • KD/KO Validated

CDK6 Monoclonal antibody, PBS Only

CDK6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4B9C11

Cat no : 66278-1-PBS

Print datasheet

Synonyms

EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6, CDKN6

验证数据展示

  • WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66278-1-Ig (same clone as 66278-1-PBS)

    WB analysis of HEK-293 using 66278-1-Ig (same clone as 66278-1-PBS)

    WB result of CDK6 antibody (66278-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HEK-293 cells. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 66278-1-Ig (same clone as 66278-1-PBS)

    IHC staining of human skin cancer using 66278-1-Ig (same clone as 66278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66278-1-Ig (CDK6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK6 antibody included
  • IF Staining of HeLa using 66278-1-Ig (same clone as 66278-1-PBS)

    IF Staining of HeLa using 66278-1-Ig (same clone as 66278-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66278-1-Ig(CDK6 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CDK6 Ready-To-Use IHC Kit. CDK6 primary antibody included.

产品信息

66278-1-PBS targets CDK6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CDK6 fusion protein Ag5600 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cyclin-dependent kinase 6
Synonyms EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6, CDKN6
Calculated Molecular Weight 326 aa, 36 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC027989
Gene Symbol CDK6
Gene ID (NCBI) 1021
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00534
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).