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验证数据展示

  • WB analysis using 83976-3-RR

    WB analysis using 83976-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83976-3-RR (CDK7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83976-3-RR

    IF Staining of HepG2 using 83976-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK7 antibody (83976-3-RR, Clone: 240962F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83976-3-RR

    Affinity and Kinetic Characterization of 83976-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83976-3-RR against Human CDK7 were performed. The affinity constant is 28.7 nM.

CDK7 Recombinant antibody

CDK7 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83976-3-RR

产品说明书

CloneNo. 240962F9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CDK-activating kinase 1, CDK activating kinase, CAK1, CAK, 39 kDa protein kinase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CDK7 Ready-To-Use IHC Kit. CDK7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83976-3-RR targets CDK7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CDK7 fusion protein Ag25737 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 7
别名 CDK-activating kinase 1, CDK activating kinase, CAK1, CAK, 39 kDa protein kinase
计算分子量 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC005298
基因名称 CDK7
Gene ID (NCBI) 1022
RRIDAB_3671551
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP50613
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDK7 antibody 83976-3-RRDownload protocol
IF protocol for CDK7 antibody 83976-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols