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  • Featured Product
  • KD/KO Validated

CDK8 Polyclonal antibody

CDK8 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 22067-1-AP

Print datasheet

Synonyms

Mediator complex subunit CDK8, K35, EC:2.7.11.22, Cyclin-dependent kinase 8, Cyclin dependent kinase 8

验证数据展示

  • WB analysis using 22067-1-AP

    WB analysis using 22067-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 22067-1-AP

    IHC staining of human stomach cancer using 22067-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human stomach cancer using 22067-1-AP

    IHC staining of human stomach cancer using 22067-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of NIH/3T3 using 22067-1-AP

    IF Staining of NIH/3T3 using 22067-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 22067-1-AP (CDK8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of NIH/3T3 using 22067-1-AP

    FC experiment of NIH/3T3 using 22067-1-AP

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug CDK8 Polyclonal antibody (22067-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells, mouse embryo tissue
Positive IHC detected inhuman stomach cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22067-1-AP targets CDK8 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen CDK8 fusion protein Ag17148 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase 8
Synonyms Mediator complex subunit CDK8, K35, EC:2.7.11.22, Cyclin-dependent kinase 8, Cyclin dependent kinase 8
Calculated Molecular Weight 464 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC069634
Gene Symbol CDK8
Gene ID (NCBI) 1024
RRIDAB_2878983
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49336
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDK8 antibody 22067-1-APDownload protocol
IHC protocol for CDK8 antibody 22067-1-APDownload protocol
IF protocol for CDK8 antibody 22067-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells.

Authors - Lindsey M Jackson
  • KD Validated
View Article
humanWB

PLoS Genet

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

Authors - Emily M Schleicher
  • KD Validated
View Article
humanWB

Biochim Biophys Acta Gene Regul Mech

PP2A and its adapter protein IER5 induce the DNA-binding ability and target gene expression of E2F1 via dephosphorylation at serine 375

Authors - Hiroto Takeuchi
View Article
mouseWB,IHC

Bull Exp Biol Med

Knockdown of Long Noncoding RNA CCAT2 Suppresses Malignant Phenotype in Human Laryngeal Squamous Cell Carcinoma

Authors - M Li
View Article