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验证数据展示

  • WB analysis using 83662-2-RR (same clone as 83662-2-PBS)

    WB analysis using 83662-2-RR (same clone as 83662-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83662-2-RR (CDK9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83662-2-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 83662-2-RR (same clone as 83662-2-PBS)

    IHC staining of human lung cancer using 83662-2-RR (same clone as 83662-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83662-2-RR (CDK9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83662-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK9 antibody included
  • FC experiment of HeLa using 83662-2-RR (same clone as 83662-2-PBS)

    FC experiment of HeLa using 83662-2-RR (same clone as 83662-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK9 Recombinant antibody (83662-2-RR, Clone:240600E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83662-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00630-4

    Sandwich ELISA standard curve of MP00630-4

    Sandwich ELISA standard curve of MP00630-4, Human CDK9 Recombinant Matched Antibody Pair - PBS only. 83662-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2318. 83662-2-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

  • Sample test of MP00630-4

    Sample test of MP00630-4

    The mean CDK9 concentration was determined to be 5,413.5 pg/mL in HEK-293T cell extract based on a 5.8 mg/mL extract load and 5,349.7 Pg/mL in Jurkat cell extract based on a 1.5 mg/mL extract load.

  • IF Staining of HeLa using 83662-2-RR (same clone as 83662-2-PBS)

    IF Staining of HeLa using 83662-2-RR (same clone as 83662-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-2-RR, Clone: 240600E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83662-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00630-2

    Cytometric bead array standard curve of MP00630-2

    Cytometric bead array standard curve of MP00630-2, CDK9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83662-5-PBS. Detection antibody: 83662-2-PBS. Standard: Ag2318. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 83662-2-RR

    Affinity and Kinetic Characterization of 83662-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83662-2-RR against Human CDK9 were performed. The affinity constant is 0.305 nM.

CDK9 Recombinant antibody, PBS Only (Detector)

CDK9 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83662-2-PBS

产品说明书

CloneNo. 240600E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

240600E3, C 2k, C-2K, CDC2L4, Cell division cycle 2-like protein kinase 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CDK9 Ready-To-Use IHC Kit. CDK9 primary antibody included.

产品信息

83662-2-PBS targets CDK9 as part of a matched antibody pair:

MP00630-2: 83662-5-PBS capture and 83662-2-PBS detection (validated in Cytometric bead array)

MP00630-4: 83662-6-PBS capture and 83662-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 CDK9 fusion protein Ag2318 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 9
别名 240600E3, C 2k, C-2K, CDC2L4, Cell division cycle 2-like protein kinase 4
计算分子量 372 aa, 43 kDa
观测分子量 38-42 kDa, 55 kDa
GenBank蛋白编号BC001968
基因名称 CDK9
Gene ID (NCBI) 1025
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50750
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).