CDKL5 Polyclonal antibody

CDKL5 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12973-1-AP

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Synonyms

CDKL5, cyclin dependent kinase like 5, ISSX, STK9



经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, PC-12 cells, SH-SY5Y cells, HeLa cell
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12973-1-AP targets CDKL5 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen CDKL5 fusion protein Ag3531 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase-like 5
Synonyms CDKL5, cyclin dependent kinase like 5, ISSX, STK9
Calculated Molecular Weight 1030 aa, 114 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC036091
Gene Symbol CDKL5
Gene ID (NCBI) 6792
RRIDAB_2877899
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO76039
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin dependent kinase like 5 (CDKL5) may regulate ciliogenesis and neuronal morphogenesis. It has been reported that CDKL5 is associated with epileptic seizures, severe intellectual disability, and autistic features. The CDKL5 protein is a kinase that regulates key phosphorylation events vital to the development of the complex neuronal network of the brain.

实验方案

Product Specific Protocols
WB protocol for CDKL5 antibody 12973-1-APDownload protocol
IP protocol for CDKL5 antibody 12973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Neurosci Res

Phenotypic characterization of Cdkl5-knockdown neurons establishes elongated cilia as a functional assay for CDKL5 Deficiency Disorder.

Authors - Alessia Di Nardo
humanWB

Cell Rep

A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder

Authors - Hanzhe Kuang