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p16INK4a Polyclonal antibody

p16INK4a Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 28416-1-AP

Print datasheet

Synonyms

Arf, ARF INK4a, Cdkn2a, INK4a ARF, Ink4a/Arf, MTS1, p16, p16(INK4a), p16INK4a, Pctr1

验证数据展示

  • WB analysis using 28416-1-AP

    WB analysis using 28416-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28416-1-AP (Cdkn2a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, C6 cells, mouse uterus tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28416-1-AP targets p16INK4a in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen p16INK4a fusion protein Ag28927 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase inhibitor 2A
Synonyms Arf, ARF INK4a, Cdkn2a, INK4a ARF, Ink4a/Arf, MTS1, p16, p16(INK4a), p16INK4a, Pctr1
Calculated Molecular Weight 18 kDa
Observed Molecular Weight19 kDa
GenBank Accession NumberNM_001040654
Gene Symbol Cdkn2a
Gene ID (NCBI) 12578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ64364
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cdkn2a (cyclin-dependent kinase inhibitor 2a) is a cell cycle regulating gene encoding alternate proteins, p16Ink4a and p19Arf, that arrest the G1 phase through CDK4/6 inhibition. As they are tumor suppressors, p16Ink4a and p19Arf can be targeted for treatment of various cancers, including melanoma, cervical, and esophageal cancers.

实验方案

Product Specific Protocols
WB protocol for p16INK4a antibody 28416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols