p16INK4a Polyclonal antibody
p16INK4a Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, C6 cells, mouse uterus tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28416-1-AP targets p16INK4a in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | mouse, rat |
| Immunogen | p16INK4a fusion protein Ag28927 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cyclin-dependent kinase inhibitor 2A |
| Synonyms | Cdkn2a, p16-INK4a, p16-INK4, p16(INK4a), p16 |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | NM_001040654 |
| Gene Symbol | Cdkn2a |
| Gene ID (NCBI) | 12578 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51480 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cdkn2a (cyclin-dependent kinase inhibitor 2a) is a cell cycle regulating gene encoding alternate proteins, p16Ink4a and p19Arf, that arrest the G1 phase through CDK4/6 inhibition. As they are tumor suppressors, p16Ink4a and p19Arf can be targeted for treatment of various cancers, including melanoma, cervical, and esophageal cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for p16INK4a antibody 28416-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
