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CDKN2AIP Polyclonal antibody

CDKN2AIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16615-1-AP

Print datasheet

Synonyms

CARF, CDKN2A interacting protein, CDKN2AIP, Collaborator of ARF

验证数据展示

  • WB analysis using 16615-1-AP

    WB analysis using 16615-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16615-1-AP

    IHC staining of human kidney using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 16615-1-AP

    IF Staining of HepG2 using 16615-1-AP

    Immunofluorescent analysis of HepG2 cells, using 16615-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HeLa using 16615-1-AP

    IP experiment of HeLa using 16615-1-AP

    IP result of anti-CDKN2AIP (IP:16615-1-AP, 4ug; Detection:16615-1-AP 1:500) with HeLa cells lysate 2800ug.

经过测试的应用

Positive WB detected inCOLO 320 cells, Jurkat cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human liver tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16615-1-AP targets CDKN2AIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen CDKN2AIP fusion protein Ag9907 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CDKN2A interacting protein
Synonyms CARF, CDKN2A interacting protein, CDKN2AIP, Collaborator of ARF
Calculated Molecular Weight 579 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC022270
Gene Symbol CDKN2AIP
Gene ID (NCBI) 55602
RRIDAB_2244775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDKN2AIP antibody 16615-1-APDownload protocol
IHC protocol for CDKN2AIP antibody 16615-1-APDownload protocol
IF protocol for CDKN2AIP antibody 16615-1-APDownload protocol
IP protocol for CDKN2AIP antibody 16615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC,IF

Cell Biosci

CDKN2AIP is critical for spermiogenesis and germ cell development

Authors - Yuming Cao
View Article
humanWB

Clin Cosmet Investig Dermatol

Genome Wide Differential Expression Profiles in Nevus Sebaceous Uncovered Low Expression of CDKN2AIP and Construction of a ceRNA Network.

Authors - Xianhong Yang
View Article