验证数据展示
经过测试的应用
| Positive IP detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
产品信息
12877-1-AP targets p15 INK4b in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | IP, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | p15 INK4b fusion protein Ag3557 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) |
| Synonyms | MTS2, CDKN2B, p14 INK4b, MTS-2, MTS 2 |
| Calculated Molecular Weight | 138 aa, 15 kDa |
| GenBank Accession Number | BC014469 |
| Gene Symbol | p15 INK4b/CDKN2B |
| Gene ID (NCBI) | 1030 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42772 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for p15 INK4b antibody 12877-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene CDKN2B deletion is essential for pancreatic cancer development instead of unmeaningful co-deletion due to juxtaposition to CDKN2A. | ||
EMBO Rep MYC promotes cancer progression by modulating m6 A modifications to suppress target gene translation. | ||
Cell Cycle Cell proliferation downregulated by TGF-β2-triggered G1/S checkpoint in clinical CAFs. | ||
Pancreatology Smad4 deficiency substitutes Cdkn2b but not Cdkn2a downregulation in pancreatic cancer following induction of genetic events in adult mice. | ||
PLoS One NRF2-regulated cell cycle arrest at early stage of oxidative stress response mechanism. | ||
Dis Model Mech A novel pancreatic cancer model originated from transformation of acinar cells in adult tree shrew, a primate-like animal. |