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CDR2 Polyclonal antibody

CDR2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11611-2-AP

Print datasheet

Synonyms

CDR2, CDR62, PCD17, Yo

验证数据展示

  • WB analysis of human brain using 11611-2-AP

    WB analysis of human brain using 11611-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11611-2-AP (CDR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 11611-2-AP

    IHC staining of human breast cancer using 11611-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11611-2-AP (CDR2 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inhuman brain tissue, Y79 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11611-2-AP targets CDR2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen CDR2 fusion protein Ag2183 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cerebellar degeneration-related protein 2, 62kDa
Synonyms CDR2, CDR62, PCD17, Yo
Calculated Molecular Weight 454 aa, 52 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC017503
Gene Symbol CDR2
Gene ID (NCBI) 1039
RRIDAB_2076734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01850
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Patients with paraneoplastic cerebellar degeneration (PCD) carry a characteristic antibody called anti-Yo. On Western blot analysis of Purkinje cells and tumor tissue from CD patient, the anti-Yo sera react with at least 2 antigens, a major species of 62 kD called CDR62 or CDR2 [PMID:18045792]. CDR2 is partly characterized, as CDR2 through its leucine zipper motif has been demonstrated to interact with c-myc, with cell cycle-related proteins and with a protein kinase, indicating that CDR2 is involved in signal transduction and gene transcription. Furthermore, CDR2 has been found to attenuate hypoxic response in renal cell carcinoma, and recent data suggest a role for CDR2 and c-myc in mitosis in cycling cells [PMID:21080165].

实验方案

Product Specific Protocols
WB protocol for CDR2 antibody 11611-2-APDownload protocol
IHC protocol for CDR2 antibody 11611-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP, WB

Acta Neuropathol

Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis.

Authors - Manja Schubert
View Article