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  • Featured Product
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CDR2L Monoclonal antibody

CDR2L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2E12D10

Cat no : 66791-1-Ig

Print datasheet

Synonyms

CDR2L, HUMPPA, Paraneoplastic 62 kDa antigen

验证数据展示

  • WB analysis of U-251 using 66791-1-Ig

    WB analysis of U-251 using 66791-1-Ig

    U-251 cells were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 66791-1-Ig

    WB analysis of rat brain using 66791-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 66791-1-Ig

    IHC staining of human cerebellum using 66791-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66791-1-Ig (CDR2L antibody) at dilution of 1:400 (under 10x lens).

    Ready-to-use IHC kits available with CDR2L antibody included
  • IHC staining of human cerebellum using 66791-1-Ig

    IHC staining of human cerebellum using 66791-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66791-1-Ig (CDR2L antibody) at dilution of 1:400 (under 40x lens).

    Ready-to-use IHC kits available with CDR2L antibody included
  • IF Staining of HEK-293 using 66791-1-Ig

    IF Staining of HEK-293 using 66791-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CDR2L antibody (66791-1-Ig, Clone: 2E12D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of HEK-293 using 66791-1-Ig

    WB analysis of HEK-293 using 66791-1-Ig

    WB result of CDR2L antibody (66791-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDR2L transfected HEK-293 cells.

经过测试的应用

Positive WB detected inU-251 cells, HEK-293 cells, K-562 cells, human brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CDR2L Ready-To-Use IHC Kit. CDR2L primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IPSee 1 publications below

产品信息

66791-1-Ig targets CDR2L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIP
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Immunogen CDR2L fusion protein Ag6139 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cerebellar degeneration-related protein 2-like
Synonyms CDR2L, HUMPPA, Paraneoplastic 62 kDa antigen
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC047534
Gene Symbol CDR2L
Gene ID (NCBI) 30850
RRIDAB_2882135
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86X02
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cerebellar degeneration-related protein 2-like(CDR2L), also named as HUMPPA or Paraneoplastic 62 kDa antigen, is a 465 amino acid protein, which belongs to the CDR2 family. CDR2L contains three potential coiled-coil regions. The functions of protein is so far not known. CDR2L is expressed in the brain and localizes in the cytoplasm.

实验方案

Product Specific Protocols
WB protocol for CDR2L antibody 66791-1-IgDownload protocol
IHC protocol for CDR2L antibody 66791-1-IgDownload protocol
IF protocol for CDR2L antibody 66791-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Ann Clin Transl Neurol

Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration.

Authors - Ida Herdlevaer
View Article