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CDX2 Polyclonal antibody

CDX2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22101-1-AP

Print datasheet

Synonyms

caudal type homeobox 2, Caudal type homeobox protein 2, CDX 3, CDX2, CDX3, Homeobox protein CDX 2

验证数据展示

  • WB analysis using 22101-1-AP

    WB analysis using 22101-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22101-1-AP (CDX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 22101-1-AP

    WB analysis using 22101-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22101-1-AP (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 22101-1-AP

    IHC staining of mouse small intestine using 22101-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of mouse small intestine using 22101-1-AP

    IHC staining of mouse small intestine using 22101-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of human appendicitis using 22101-1-AP

    IHC staining of human appendicitis using 22101-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDX2 antibody included
  • IF Staining of Caco-2 using 22101-1-AP

    IF Staining of Caco-2 using 22101-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (22101-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

经过测试的应用

Positive WB detected inCOLO 320 cells, Caco-2 cells, mouse colon tissue, mouse brain tissue
Positive IHC detected inmouse small intestine tissue, human appendicitis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells
Planning an IHC experiment? We recommend our IHCeasy CDX2 Ready-To-Use IHC Kit. CDX2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22101-1-AP targets CDX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CDX2 fusion protein Ag17310 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caudal type homeobox 2
Synonyms caudal type homeobox 2, Caudal type homeobox protein 2, CDX 3, CDX2, CDX3, Homeobox protein CDX 2
Calculated Molecular Weight 313 aa, 34 kDa
Observed Molecular Weight 33-40 kDa
GenBank Accession NumberBC014461
Gene Symbol CDX2
Gene ID (NCBI) 1045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99626
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

实验方案

Product Specific Protocols
WB protocol for CDX2 antibody 22101-1-APDownload protocol
IHC protocol for CDX2 antibody 22101-1-APDownload protocol
IF protocol for CDX2 antibody 22101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols