验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, mouse testis tissue, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17763-1-AP targets CDYL in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CDYL fusion protein Ag12143 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromodomain protein, Y-like |
| 别名 | CDY like, CDYL, CDYL1, chromodomain protein, Y like, Chromodomain Y like protein |
| 计算分子量 | 544 aa, 61 kDa |
| 观测分子量 | 66 kDa, 60 kDa |
| GenBank蛋白编号 | BC108725 |
| 基因名称 | CDYL |
| Gene ID (NCBI) | 9425 |
| RRID | AB_2077197 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y232 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDYL antibody 17763-1-AP | Download protocol |
| IHC protocol for CDYL antibody 17763-1-AP | Download protocol |
| IP protocol for CDYL antibody 17763-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Endocrinology Dynamic profiles and transcriptional preferences of histone modifications during spermiogenesis. | ||
Adv Sci (Weinh) Cdyl Deficiency Brakes Neuronal Excitability and Nociception through Promoting Kcnb1 Transcription in Peripheral Sensory Neurons. | ||
Drug Des Devel Ther Sorafenib Attenuates Fibrotic Hepatic Injury Through Mediating Lysine Crotonylation | ||
Cancer Lett CDYL knockdown reduces glioma development through an antitumor immune response in the tumor microenvironment
| ||
Acta Pharmacol Sin Chromodomain Y-like (CDYL) inhibition ameliorates acute kidney injury in mice by regulating tubular pyroptosis | ||
Cell Rep The chromodomain protein CDYL confers forebrain identity to human cortical organoids by inhibiting neuronatin
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