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CEA/CD66e Monoclonal antibody

CEA/CD66e Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B8H6

Cat no : 68377-1-Ig

Print datasheet

Synonyms

CEA, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen

验证数据展示

  • IHC staining of human colon cancer using 68377-1-Ig

    IHC staining of human colon cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human colon cancer using 68377-1-Ig

    IHC staining of human colon cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • WB analysis using 68377-1-Ig

    WB analysis using 68377-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68377-1-Ig

    WB analysis using 68377-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHaCaT cells, human saliva, HT-29 cells, human rectum tissue
Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CEA/CD66e Ready-To-Use IHC Kit. CEA/CD66e primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68377-1-Ig targets CEA/CD66e in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Synonyms CEA, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen
Calculated Molecular Weight 77 kDa
Observed Molecular Weight180-200 kDa
GenBank Accession NumberBC034671
Gene Symbol CEA
Gene ID (NCBI) 1048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06731
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.

实验方案

Product Specific Protocols
WB protocol for CEA/CD66e antibody 68377-1-IgDownload protocol
IHC protocol for CEA/CD66e antibody 68377-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols